You will find below a description of the current services provided by GeneCust. If the service you need is not listed below, please feel free to contact us to discuss your project.

Complete service including cDNA cloning, recombinant adenovirus construction, amplification and purification is available.

Optionnal tests available :

• PCR assay for replication-competent adenovirus (RCA).
• Assay for replication-competent adenovirus (RCA) using A549 cells.
• TCID50

services- Insert a validated RNAi sequence into an adenoviral vector. Also available , design and validation of SiRNA.

SiRNA-Ad Service Package is a complete service of RNAi selection, validation, adenoviral construction, amplification and purification (3x10^12 vp) at a reduced price. Feel free to request a quote.

SiRNA Synthesis

All oligos undergo vigorous process monitoring and stringent quality control; they are produced under ISO9000 quality standard system and HPLC purified so that purity SiRNA > 97%.

Products are quantified by spectrophotometer. Oligonucleotides are delivered with an Oligonucleotide Technical Data Sheet, which includes oligo name, sequence, concentration, orecise quantity in OD and nmols, Tm, MW, size extinction coefficient and putificatiion data.

Chemical synthesis, FAM labelling, positive and negative controls available.

services - RNAi Set.

Chemically synthetic SiRNA became rapid and effective method for gene knockdown. We recently promoted RNAi set service. You provide only gene (target) sequence and we help you to design 4 SiRNA pairs.

We guarantee at least one pair can effectively suppress gene expression. The RNAi Sets Includes :

• 4 pairs of corresponding gene SiRNA, HPLC purification, 10 nmol
• Negative control, 1 pair, HPLC purification, 1 OD
• Negative control, FAM labelled SiRNA, 1 pair, HPLC purification, 1 OD
• Positive control GAPDH SiRNA, 1 pair, HPLC purification, 1 OD
• Transfection reagent RNAi-mate, 100 µl

services - RNAi Mate transfection reagent.

RNAi Mate transfection reagents can be used in vivo and in vitro for nucleic acid including DNA, RNA, antisense oligo and SiRNA, and also can be used in co-transfection for DNA/SiRNA.

The Commercially available SiRNA transfection reagents can not meet the needs for high-throughput SiRNA transfection experiments. Therefore, transfection reagents Cation-liposome "RNAi Mate" was developped, which is highly efficient compared to the market-meading reagent.

services - Vector based RNAi, Custom cloning.

GeneCust provides a set of vectors includong shRNA expression vectors, as well as corresponding negative control vector, GFP positive control vector.

We may help you to construct plasmid with insertion of targeted shRNA, and will offer accurate sequence control. You only have to tell us the target gene sequence or Gene ID number of insertion vector name, and we will send you the construct. We will provide highly purified shRNA expression vector.

GeneCust can synthesize almost any peptide you requested. Whether your peptide is short or long, we can synthesize it for you. For short custom peptides (<50-mer), we can synthesize them using our chemical method. For long custom peptides (>50-mer), we can synthesize them using our proprietary recombinant technology.

Every single custome peptide from GeneCust is analyzed by Mass spectrum and HPLC. The quality control data will be provided to the customer. For most peptides, our typical turnaround time is about 3 weeks. The delivery time may vary depends on the peptide length and difficulty. GeneCust custom peptides synthesis service is competitively priced.

GeneCust provides a time-tested, high-quality service standard. Comprehensive service package includes antigen design, peptide synthesis, polyclonal antibody and monoclonal antibody production. Rat monoclonal or polyclonal antibodies for the study of gene expression and proteomics in mice and in mouse models or human diseases are available.

We will carry out artificial gene synthesis up to 20 kb, codon optimization for free if required and double strand DNA sequencing on final product. You will receive 4 µg of your artificial gene inserted into pUC57 or pBluescriptIISK+ plasmid for free. Our large production capacity (600-800 genes per month) garantee our fast turnaround.

Discover our "gene mutant" associated service. Order one gene at price list and get its variants for only 200 euros !

Combined with high efficiency gene synthesis service, GeneCust Europe has produced a large human and mouse ORF database. All ready ORF clones are provided in a pEXCMV expression vector, thus it is ready to use and transfect. Each Ready ORF clone has been full-insert sequenced and each clone, before delivery, is sequenced again to ensure 100% accuracy.

You will receive with each ordered clone, complete QC data including chromatograms and vector description. We provide 5 µg TE dissolved DNA then you can easily expend your clone.

You just have to send us an email with accesion number or ID and we will send you very shortly a specific quote. All clones have only one price : 990 euros !

• Human or mouse ORF cloned into expression vector pEXMCV
• Ready to use and transfect clones supplied
• Guaranteed sequence, data available
• Delivery of 5 µg DNA
• One price for all clones whatever length or complexity

This fully customizable service can be used either for cloning genes, cDNA, PCR products or for subcloning any plasmid insertion into any vector. You provide us with accession number or any product and we will do the rest.

You will received the purified plasmid and the results of the restriction enzyme analysis for your plasmid.

We will carry out site directed mutagenesis on your DNA sequence to make specified base changes. Our technology ensures very high mutation rates.

The service include design, synthesis of mutagenic oligonucleotides, mutagenesis, transformation, plasmid DNA isolation and double strand corrected sequencing.

Our high quality is not only guaranteed by computer-assisted trityl monitoring during synthesis, but also by a quality control that assesses every single Oligo. All Oligos are analyzed by gel electrophoresis before shipment.

We offer a complete range of re-furbished instruments for DNA and Protein Analysis. Our instruments are re-furbished and come with a warranty, unless otherwise started

We provide service support for many of the instrument systems we sell. This includes systems which are no longer supported by the original manufacturer.

We can support you with :

• Preventive Maintenance
• System re-furbishment
• Comprehensive Annual Repair Contracts
• Service Call Outs with hourly rates

For details, of pricing or to discuss your requirements further please contact us directly.

We provide now consumables for ABI DNA Analyzers : polymers, BigDye kits, capillaries.

DNA Synthesis Products : Chemgenes

This USA company is known worldwide in molecular biology and offers special products for DNA synthesis. Its catalog contains several hundred references. GeneCust is the European retailer for the Chemgenes product range.

Peptide Synthesis Products : check our products range

• N-Protecting Reagents
• Peptide Coupling Reagents
• Linkers for Solid Phase Synthesis
• Other Reagents
• Amino Acids and Derivatives
• Unusual Amino Acids
• N-Methyl Amino Acids
• Boc-Amino Acids and Derivatives
• Fmoc-Amino Acids and Derivatives
• Z-Amino Acids and Derivatives
• Amino Alcohols
• Fmoc-Amino Acids attached to Wang Resin
• Amino Acids 2-Chlorotrityl Resin
• Other Resin and Derivatives